Isolation of Proteinase K-Sensitive Prions Using Pronase E and Phosphotungstic Acid
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چکیده
منابع مشابه
Isolation of Proteinase K-Sensitive Prions Using Pronase E and Phosphotungstic Acid
Disease-related prion protein, PrP(Sc), is classically distinguished from its normal cellular precursor, PrP(C), by its detergent insolubility and partial resistance to proteolysis. Molecular diagnosis of prion disease typically relies upon detection of protease-resistant fragments of PrP(Sc) using proteinase K, however it is now apparent that the majority of disease-related PrP and indeed prio...
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Oxidative damage is thought to play a role in the aetiology of aging and a number of diseases including cancer, chronic inflammation, ischemia, degenerative arterial and autoimmune diseases [I]. 8-oxodeoxyguanosine (8-oxodG), an oxidative DNA adduct. has gained much popularity as a biomarker of damage to DNA. HPLC combined with electrochemical detection provides a selective and sensitive method...
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Prions arise when the cellular prion protein (PrP(C)) undergoes a self-propagating conformational change; the resulting infectious conformer is designated PrP(Sc). Frequently, PrP(Sc) is protease-resistant but protease-sensitive (s) prions have been isolated in humans and other animals. We report here that protease-sensitive, synthetic prions were generated in vitro during polymerization of rec...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2010
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0015679